The First Round draw is seeded based on the final league positions of teams in the 2013/14 season, and then split regionally between north and south.
North - seeds
1. Barnsley
2. Blackburn Rovers
3. Blackpool
4. Bolton Wanderers
5. Bradford City
6. Derby County
7. Doncaster Rovers
8. Huddersfield Town
9. Leeds United
10. Middlesbrough
11. Nottingham Forest
12. Port Vale
13. Preston North End
14. Rotherham United
15. Sheffield United
16. Sheffield Wednesday
17. Wigan Athletic
North - non-seeds
18. Accrington Stanley
19. Burton Albion
20. Bury
21. Carlisle United
22. Chesterfield
23. Crewe Alexandra
24. Fleetwood Town
25. Hartlepool United
26. Mansfield Town
27. Morecambe
28. Notts County
29. Oldham Athletic
30. Rochdale
31. Scunthorpe United
32. Shrewsbury Town
33. Tranmere Rovers
34. York City