The draw will be conducted by former Tottenham captain Ledley King and former Bolton midfielder Fabrice Muamba
The draw numbers are as follows:
| 1 | Arsenal |
| 2 | Aston Villa |
| 3 | Barnsley |
| 4 | Birmingham |
| 5 | Blackburn |
| 6 | Blackpool |
| 7 | Bolton |
| 8 | Brighton |
| 9 | Bristol City |
| 10 | Burnley |
| 11 | Cardiff |
| 12 | Charlton |
| 13 | Chelsea |
| 14 | Crystal Palace |
| 15 | Derby |
| 16 | Everton |
| 17 | Fulham |
| 18 | Huddersfield |
| 19 | Hull City |
| 20 | Ipswich |
| 21 | Leeds |
| 22 | Leicester |
| 23 | Liverpool |
| 24 | Manchester City |
| 25 | Manchester United |
| 26 | Middlesbrough |
| 27 | Millwall |
| 28 | Newcastle |
| 29 | Norwich |
| 30 | Nottingham Forest |
| 31 | Peterborough |
| 32 | Queens Park Rangers |
| 33 | Reading |
| 34 | Sheffield Wednesday |
| 35 | Southampton |
| 36 | Stoke City |
| 37 | Sunderland |
| 38 | Swansea |
| 39 | Tottenham |
| 40 | Watford |
| 41 | West Brom |
| 42 | West Ham |
| 43 | Wigan Athletic |
| 44 | Wolves |
| 45 | Preston |
| 46 | Bury or Southend |
| 47 | Sheffield United |
| 48 | AFC Bournemouth |
| 49 | Burton Albion |
| 50 | Bradford City or Brentford |
| 51 | Luton Town |
| 52 | Alfreton Town or Leyton Orient |
| 53 | Oldham |
| 54 | Tranmere |
| 55 | Rotherham or Notts County |
| 56 | Cheltenham or Hereford |
| 57 | Barrow or Macclesfield Town |
| 58 | Accrington Stanley or Oxford United |
| 59 | Lincoln City or Mansfield |
| 60 | Harrogate Town or Hastings United |
| 61 | Coventry |
| 62 | Milton Keynes Dons or AFC Wimbledon |
| 63 | Crawley Town |
| 64 | Aldershot Town |